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M94A3315.TXT
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1994-10-25
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Document 3315
DOCN M94A3315
TI Distinct mechanisms of HIV-1 latency in the two cell line models U1 and
ACH-2.
DT 9412
AU Cannon P; Kim SH; Ulich C; Kim S; Seoul National University, Korea.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):104 (abstract no. PA0035). Unique
Identifier : AIDSLINE ICA10/94369260
AB The U1 and ACH-2 cell lines are human monocytic and T-lymphoid cells
respectively, persistently infected with HIV-1. These cell lines express
viral RNA and proteins at very low levels, but can be induced to produce
larger amounts virion particles. For this reason, they have been used
extensively as in vitro models of HIV latency. In order to examine the
basis for the latent state of HIV in these cell lines, we have
investigated the roles of the essential viral regulatory factor Tat.
First, we transiently transfected the HIV LTR as a reporter plasmid for
Tat function. Second, purified Tat protein was added to the culture to
test whether HIV production could be increased. Third, we constructed U1
and ACH-2 derivatives constitutively expressing Tat. Results obtained
from these analyses indicated that the provirus in the U1 cell line is
latent because of suboptimal levels of Tat, while Tat is not limiting
for viral production in ACH-2 cells. A different mechanism therefore
underlies the latent state of HIV in U1 and ACH-2 cells. This result
also argues against a model of HIV latency based simply on suboptimal
levels of the Rev protein. We are currently studying the role(s) of Rev
using experimental schemes similar to those employed for the analysis of
Tat.
DE Cell Line Gene Expression Gene Products, rev/GENETICS/PHYSIOLOGY Gene
Products, tat/GENETICS/PHYSIOLOGY Genes, tat Human HIV
Infections/ETIOLOGY HIV Long Terminal Repeat
HIV-1/GENETICS/*PHYSIOLOGY/PATHOGENICITY Models, Biological
Monocytes/MICROBIOLOGY T-Lymphocytes/MICROBIOLOGY Transfection Virus
Replication/GENETICS/PHYSIOLOGY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).